Study on Tissue Culture of Dendrobium candidum by Tissue Culture Culture Room

The medicinal value of Dendrobium candidum is of great concern and its ornamental value is also high. However, due to its tiny seed and no endosperm, it needs to be symbiotic with certain fungi to germinate under wild conditions. It is difficult to adopt seedling cultivation, while the traditional ramets, cuttings and other methods have extremely low reproduction rates, and over-excavation, Pre-existing people have used tissue culture techniques to achieve certain achievements in the preservation and rapid propagation of D. candidum. However, after several generations of fine varieties, or due to inappropriate use of hormones, the cultivation of the environment and other factors, the tissue culture seedlings are prone to occur. Degradation, even jeopardizes the quality and scale of the entire tissue culture system. The tissue culture chamber plays an important role in tissue culture. For this reason, the use of this instrument is worth promoting.

In the tissue culture chamber, the MS medium (Co 2+ regulated in an amount of 0.02; pmg/I) supplemented with 2.0 mg/I_6-BA}O.10 mg/I_NAA was used as a control. The chlorinated drill (CoCI,"6H,O ) Prepare the mother liquor with the concentration of Q02; pmg/I separately, and take different volumes of Co} ion mother liquor to add the medium of MS}2.0mg/I.6-BA}0.10mg/I_NAA (Saccharose 300, agar 0.700, 150g /I fresh potato juice), culture medium prepared with Co} concentration of M} medium; 1.12; p, 1.2; p0, 1.; p00-fold medium; distorted tissue culture seedlings distorted with mild yellowing For the test material, after removing the root pocket, inoculate each formulation medium with 6 bottles, inoculate 1 bottle each; p strains; p0d observe and count the growth of the tissue culture seedlings, and explore the concentration of Co} on the disintegrating tissue culture seedlings of D. Rejuvenation effect.

The cultivation of Dendrobium candidum tissue culture chambers found that different types of plant hormones produce physiological effects that promote or antagonize each other and regulate the growth and development of plants through the combined action of various hormones. The type and absolute concentration of hormones depend on the proper proportion and balance of hormones.

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