Chlorophyll content determination method

Chlorophyll is a necessary substance for photosynthesis. Without chlorophyll, photosynthesis can not be performed normally, and light energy can be converted into chemical energy. Chlorophyll can be found in green plants, blue-green algae and other organisms, and is divided into chlorophyll a, chlorophyll b, chlorophyll c, and chlorophyll d. Chlorophyll is present in the chloroplast vesicles.

Three Methods for Determination of Chlorophyll Content

Chlorophyll content is closely related to the photosynthetic rate and plant nutrition status. Usually, we will know the growth status of plants by measuring the chlorophyll content. In general, there are three kinds of plant chlorophyll content determination: spectrophotometry, living chlorophyll meter method and photoacoustic spectroscopy. Spectrophotometry is the most widely used method for determining chlorophyll content.

Absorption Spectrum of Leaf Chlorophyll in Different Extracts of Coral Tree

1-95% acetone 2 ethanol 2-95% ethanol

3-80% acetone 4-95% acetone 2 ethanol (homogenate)

Calculation of chlorophyll content

The determination of chlorophyll content firstly extracts the chloroplast pigment. The chloroplast pigment contains chloroplast a, chloroplast b, carotene and lutein. When extracting chloroplast pigments, we use different reagents such as acetone, ethanol and other organic substances. When the reagents are different, the results obtained will be different. In the picture above we use four different solvents for the extraction of chloroplast pigments. In the figure, the chlorophyll content was calculated by comparing the absorbance of the four solvents at wavelengths between 600 nm and 700 nm. From the figure, we can roughly conclude that the light absorption properties of the four chlorophyll extracts are basically the same.

The determination of chlorophyll content by spectrophotometry is based on the Lambert-Beer law and the mathematical expression is A=Kbc. Among them, A is the absorbance; K is the absorption coefficient; b is the thickness of the solution; c is the concentration of the solution.

The maximum absorption peaks of the chlorophyll a, b solution in acetone in the visible range were at 663 nm and 645 nm, respectively. The absorbance coefficients of chlorophyll a and b at 663 nm were 82.04 and 9.27, respectively; the absorbance coefficients at 645 nm were 16.67 and 45.60, respectively. According to the Lambert-Beer law, we can list the equations:

D663 = 82.04Ca+9.27Cb

D645 = 16.67Ca+45.60Cb

According to the above equations, you can find:

Ca = 12.72D663 C 2.59D645

Cb = 22.88D645 C 4.67D663

Then we can find the total amount of chlorophyll CT = (D652 × 1000) / 34.5

The above figure shows the progress of different chlorophyll extracts in the extraction of chlorophyll. It can be seen that the difference is still quite large. We can compare ourselves. From the figure, we can find that 95% acetone 2 ethanol (homogeneous) has the best extraction effect. Therefore, when we carry out chlorophyll content determination, we can consider using 95% acetone 2 ethanol (homogenized) as the extract.

So, we have now invented a relatively convenient instrument for measuring chlorophyll content—the chlorophyll content measuring instrument. It measures the performance index of soil and the nitro requirement of plants by measuring the relative chlorophyll content of plants, and understands the Growth Status.


Graph Chlorophyll Content Tester

Chlorophyll content measuring instrument technical parameters

Measuring object


Plant leaves


measurement method

2-wavelength optical density difference method

Measuring area

2mm×3mm

sensor

Silicon Semiconductor Photodiode

Display method

Measured value: 3-digit LCD display

Measurement times: 2 digit LCD display

Measuring range

0.0 to 99.9 SPAD

Memory capacity

30 data, automatically calculate and display the average

power supply

2 AA batteries (1.5V)

Battery Life

More than 20000 measurements

Measurement interval

Less than 2 seconds

Accuracy

Within ±1.0 SPAD units (SPAD values ​​range from 0-50 at room temperature)

Repeatability

Within ±0.3 SPAD units (SPAD values ​​between 0-50)

Reproducibility

Within ±0.5 SPAD units (SPAD values ​​between 0-50)

Volume (length×width×height)

164×78×49mm

weight

225 grams (excluding batteries)

Operating environment

0 to 50°C

Storage environment

-20 to 55°C

Other functions

Alarm function; calibration function

Standard configuration

Host, Calibration Card, Host Cover, Battery

Basketball

JYMINGDE has specialized in making basketball balls for 40 years.We are professional custom basketball manufacturer and we have Rubber Basketball and laminated basketball.We can custom logo,color,design.We have strong QC team.Our basket ball bladder are kept in cages for 48 hours to check air leakage problem, so no air leakage problem.


Our advantages:
a) Established in 1993, 6000-7000pcs/day production capacity, delivery time is guaranteed
b) Pass testing like CE, CPSIA, REACH, SEDEX, COSTCO, YUM audit, will do BSCI audit every year.
c) Have cooperated with Costco, Dick's Sports, Macy's, Umbro, Menards, Family Dollar, Dollarama, Pizza Hut
d) Own patent certification for LED light up balls in China. Can expand your product line and occupy the market


If you want to learn more information of us, please feel free to contact us and I will show you our pictures or video. Hoping to cooperate with you in the future.

Basketball,Olympic Basketball,Durable Basketball,Sport Basketball

Jiangyin Mingde Sports Goods Co., Ltd. , https://www.mdsportsball.com

Posted on