Leaf area analyzer for determination of leaf surface at different growth stages of lettuce

Stem lettuce is an important vegetable in China, widely grown, and the lettuce has many different varieties, but according to its leaf type there are two major types, long leaf type and oval leaf type. The leaf is an important organ for plant growth and development and is responsible for photosynthesis and transpiration, so the area of ​​the leaf is a very important indicator. The determination of leaf area is extremely important in the research of plant growth physiology and agrobiology. The leaf area is also related to the indicators of various plant growth. These indicators are often used in plant growth analysis.

Previous methods have been established to measure and estimate leaf area. These methods can be classified as destructive, non-destructive or direct and indirect. The method of measuring leaf area was discussed. Direct measurement of leaf area is limited to the application of leaf area meters. However, for a researcher without a leaf area meter, measuring the leaf area in a non-destructive way involves measuring the blade geometry.

The leaf stem lettuce leaves used in the leaf area test were used in two varieties (long-leaved “Daibai” and oval leaf “round leaves”). The two cultivars were sown on October 11th and transplanted when they grew 3-4 true leaves a month later. Then they were conventionally cultivated in the field. Randomized blocks were set up with three replicates, using two planting intervals (30cm x 30cm, 30cm x 40cm) and a cultivation plot of 25m2. Thirty strains of “two white peels” and “round leaves” (weeks 8, 16, 20, and 24) of different growth stages were randomly selected and 300 pieces of leaves of different sizes were used. The maximum length and the maximum width of each leaf were measured. Accurate to 0.1cm. The individual leaves were then measured with an LI-188b leaf area meter to an area of ​​0.1 cm2.

Through the analysis of the leaf area analyzer, statistical analysis results of leaf length, leaf width, and leaf area at different growth stages can be seen in the differences in leaf length, leaf width, and leaf area at different (or different) stages of different cultivars. Significant. The range of leaf length, leaf width and leaf area indicated that the variety and growth stage had a great influence on the leaf. Taking long leaf varieties as an example, the length and width of the leaves at the 8th week were 12.02 cm and 2.73 cm, respectively, but the length and width increased to 27.94 cm and 7.11 cm respectively at 24 weeks.

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