Corn seed purity identification reagent preparation method
First, the electrode buffer Weigh glycine 6.00g, poured into a 2000ml beaker, add about 1800ml of deionized water to dissolve, with about 2.0ml lactic acid adjusted to pH3.3, add deionized water volume to 2000ml, and mix.
Second, the sample extract was weighed 5.80g of sodium chloride, sucrose 200g, methyl green 0.15g, poured into a 1000ml beaker, add about 800ml of deionized water dissolved, heated to micro-boiling, let cool, with freshly cooked Ionic water volume to 1000ml. Store in a 40C refrigerator.
Third, the separation of glue buffer to take 1.43ml of sodium lactate in a 1000ml beaker, add about 980ml of deionized water, adjusted to pH3.0 with lactic acid, dissolved to 1000ml, stored in a brown bottle, into the 40C refrigerator.
Fourth, the separation of glue solution Weigh 112.5g of acrylamide, NN 'A methylene acrylamide 3.75g, ascorbic acid 0.25g, ferrous sulfate 8.0mg, dissolved in the separation buffer, the volume to 1000ml, filtered in a brown bottle , put it in a 40C refrigerator (not more than 2 weeks).
V. Concentrated Gel Buffer Take 0.30 ml of sodium lactate in a 200 ml beaker, add about 90 ml of deionized water, adjust to pH 5.2 with lactic acid, set the volume to 100 ml, store in a brown bottle, and store in a 40C refrigerator.
Sixth, the concentrated glue solution Weigh acrylamide 6.00g, NN '-methylene bis acrylamide 1.00g, ascorbic acid 0.03g, ferrous sulfate 0.8 mg, dissolved in a concentrated gel buffer, constant volume to 100ml, stored in a brown bottle Medium, put in 40C refrigerator (not more than 1 week).
Seven, 3% hydrogen peroxide solution Take 30% of hydrogen peroxide 1ml, plus 9ml of deionized water, stored in a brown bottle, into the 40C refrigerator.
Eight, staining solution Weigh Coomassie brilliant blue R250 2.00g, in a mortar and pestle with 100ml of anhydrous ethanol, filtered in a brown bottle. Take 10ml of this solution, add it to 200ml of 10% (w/v) trichloroacetic acid solution and mix.
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